CHAPTER- 10:BIOTECHNOLOGY AND ITS APPLICATIONS

EXERCISES

1. Which part of the plant is best suited for making virus-free plants and why?

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2. What is the major advantage of producing plants by micropropagation?

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3. Find out what the various components of the medium used for

propagation of an explant in vitro are?

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4. Crystals of Bt toxin produced by some bacteria do not kill the bacteria themselves because –

(a) bacteria are resistant to the to

(b) toxin is immature;

(c) toxin is inactive;

(d) bacteria encloses toxin in a special sac.

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5. What are transgenic bacteria? Illustrate using any one example.

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6. Compare and contrast the advantages and disadvantages of production of genetically modified crops.

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7. What are Cry proteins? Name an organism that produce it. How has man exploited this protein to his benefit?

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8. What is gene therapy? Illustrate using the example of adenosine

deaminase (ADA) deficiency.

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9. Digrammatically represent the experimental steps in cloning and

expressing an human gene (say the gene for growth hormone) into a

bacterium like E. coli ?

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10. Can you suggest a method to remove oil (hydrocarbon) from seeds based on your understanding of rDNA technology and chemistry of oil?

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11. Find out from internet what is golden rice.

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12. Does our blood have proteases and nucleases?

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13. Consult internet and find out how to make orally active protein

pharmaceutical. What is the major problem to be encountered?

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